What is HEPES buffer used for?

What is HEPES buffer used for?

Specifically, HEPES buffer is used to maintain the ideal conditions for cell culture. Therefore, it’s classed as a biological buffer and is best suited for microbiology and histology. It’s also useful in medicine as part of diagnostic laboratory procedures.

What does HEPES do in cell culture media?

HEPES may be added to cell culture media at a final concentration of 10mM to 25 mM to provide additional buffering capacity if required. Lower concentrations are usually not adequate to control fluctuations in pH, and higher concentrations are often toxic.

What HEPES stands for?

Acronym. Definition. HEPES. N-2-Hydroxyethylpiperazine-N’-2-Ethanesulfonic Acid.

Why are HEPES used in cell culture?

HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by aerobic respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture.

What are HEPES IVF?

IVF Basics® HTF HEPES is a handling, conditioning and culture medium enriched with HEPES for the use outside a CO2 incubator. IVF Basics® HTF HEPES is already used in over 100.000 IVF cycles and is developed for IVF, GIFT and ZIFT procedures in order to facilitate the human gamete and embryo manipulation.

Is HEPES buffer acidic or basic?

HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulfonic acid buffering agent, thus it has an acidic sulfonic acid group (pKa ~3) and a weakly basic piperazine group (pKa ~7.5).

What is HEPES in skincare?

Sulfonic Acid (Hepes) is a synthetically derived acid that activates natural enzymes in the skin to help break the bonds that bind dead skin cells to the surface for even exfoliation.

Are HEPES safe?

2.3 Material may be irritating to the mucous membranes and upper respiratory tract. May be harmful by inhalation, ingestion, or skin absorption. May cause eye, skin, or respiratory system irritation. To the best of our knowledge, the toxicological properties have not been thoroughly investigated.

Is HEPES a lysis buffer?

Hypotonic Lysis Buffer, (HEPES 25 mM, MgCl2 5 mM, EDTA 5 mM, DTT 5 mM) pH 7.5. Temperature sensitive items must be shipped overnight.

How does pH affect embryonic development?

Maintaining the intercellular pH (pHi) is one of the vital roles of embryo homeostasis since pHi regulates many cellular processes. Indeed, variations in pHi can affect embryonic development. Although the embryo has biochemical systems to regulate its pHi, these systems can be overtaken.

Are vitrification solutions bicarbonate buffered?

These vitrification-warming basal media utilize a bicarbonate/CO2 buffer system. The inclusion of sodium bicarbonate requires the use of a gassing incubator to maintain the physiological pH (7.2–7.4). Thus, plates containing the vitrification media must be equilibrated in the incubator prior to use.