What is the meaning of HPTLC?
HPTLC (high-performance thin layer chromatography) is a sophisticated form of TLC, which provides superior separation efficiency. The HPTLC concept includes validated methods for qualitative and quantitative analysis, and fulfills all quality requirements for use in fully regulated environments.
Why is HPTLC used?
HPTLC is used for purity control of chemicals, pesticides, steroids, and water analysis. [50] HPTLC is also widely used for analysis of vitamins, water-soluble food dyes, pesticides in fruits, vegetables, and other food stuffs.
Who invented HPTLC?
For the purpose of analyzing ezetimibe in combination with simvastatin, three HPTLC methods have been developed. The first method was developed by Shivshankar et al. [33] and yielded higher Rf values for either ezetimibe or simvastatin. In the method developed by Dhaneshwar et al.
How do you perform a HPTLC?
How HPTLC is Performed. HPTLC is not very different from TLC in terms of the underlying principle. Briefly, you apply the sample on a TLC plate and run the sample by dipping the plate in a particular solvent system. The compounds in the sample will then separate based on their polarities.
What is the difference between HPLC and HPTLC?
HPTLC is an improved version of TLC, while HPLC is a pump driven flow system with a stationary phase filled column. HPLC and HPTLC are based on similar chromatographic principles and are high performance techniques.
What is HPLC principle?
Principle of HPLC The specific intermolecular interactions between the molecules of a sample and the packing material define their time “on-column”. Hence, different constituents of a sample are eluted at different times. Thereby, the separation of the sample ingredients is achieved.
Which lamps are used in HPTLC?
TLC Scanner III:With three light sources-mercury vapour lamp, deuterium lamp, or tungsten. HPTLC is a versatile separation technique and is official in the most of the pharmacopoeias for determining content uniformity, purity profile, assay values and dissolution rates in unlimited number of monographs.
What is difference between TLC and HPTLC?
TLC and HPTLC are two chemical techniques used to separate non-volatile components in a mixture. The key difference between TLC and HPTLC is that the absorbent material in TLC plate has large particles whereas HPTLC plates have very small particles of adsorbent material. TLC is thin layer chromatography.
Which is better HPTLC or HPLC?
For qualitative analysis, HPTLC is too good technique. but for quantitative you should go for HPLC or UPLC. For herbal drugs/ crude plant extracts which have the complex mixture of many chemical constituents of various nature/ polarity, HPTLC being the open system is better than HPLC.
What is the feature of HPTLC?
FEATURES OF HPTLC: Lower analysis time and less cost per analysis. Low maintenance cost. Visual detection possible – as it is an open system. Availability of a great range of stationary phases with unique selectivity for mixture components.