Live truth instead of professing it

What is M13 primer sequence?

What is M13 primer sequence?

The pUC/M13 Primers are used to sequence inserts cloned into the M13 vectors and pUC plasmids developed by Messing. The primers are purified by gel electrophoresis or HPLC and supplied in sterile water.

What is pGL3?

The pGL3 Luciferase Reporter Vectors provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These may be cis- or trans-acting factors.

What is M13 sequencing?

The dideoxy chain terminator/Ml3 vector method of deoxyribonucleic acid (DNA) sequencing is considered to be the fastest method to determine the sequence of large fragments of DNA. Lengths of DNA are cloned into the bacteriophage M13 that provides a source of large quantities of single-stranded DNA.

What is hRluc?

75[hRluc/CMV] Vector encodes the luciferase reporter gene hRluc (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression.

Why are primers used in PCR?

PCR (Polymerase Chain Reaction) Because DNA polymerase can add a nucleotide only onto a preexisting 3′-OH group, it needs a primer to which it can add the first nucleotide. This requirement makes it possible to delineate a specific region of template sequence that the researcher wants to amplify.

Do primers start at 3 or 5?

Generally, primers are between 15 and 21 base pairs in length. First we need to understand how DNA is copied. DNA is amplified from the 3′(pronounced Three Prime) end to the 5′(pronounced Five Prime) end of the strand being copied. The amplification is said to go in the 5′ to 3′ direction.

Why is M13 used for sequencing?

M13 is the vector of choice for dideoxy sequencing for two main reasons. First, M13 bacteriophages are packaged in single strands of DNA, which are extruded from infected Escherichia coli cells into the surrounding culture medium.