What kind of structure does Barnase have?
The calculated structures contain two alpha-helices (residues 6-18 and 26-34) and a five-stranded antiparallel beta-sheet (residues 50-55, 70-75, 85-91, 94-101, and 105-108). The core of the protein is formed by the packing of one of the alpha-helices (residues 6-18) onto the beta-sheet.
What is Barnase Barstar?
The barnase-barstar transgene based hybrid seed production technology has been commercially deployed in crops like Brassica napus (Mariani et al. 1990; 1992). The system utilizes the expression of barnase gene, encoding cytotoxic RNase protein in the tapetum cells to make one of the combiner’s male sterile.
What is the biological function of Barnase?
Recoverable block of function Barnase encodes a potent ribonuclease, which when expressed, kills a cell because it chews up the whole protein manufacturing system.
What is the full name of the 8th amino acid in Barnase?
Barnase (a portmanteau of “BActerial” “RiboNucleASE”) is a bacterial protein that consists of 110 amino acids and has ribonuclease activity. It is synthesized and secreted by the bacterium Bacillus amyloliquefaciens, but is lethal to the cell when expressed without its inhibitor barstar….Barnase.
What is the full name of the 8th amino acid in barnase?
What is transgenic male sterility?
Transgenic male sterility is controlled by foreign nuclear genes. In this system two types of transgenes, viz., barnase and barstar are used. The gene barnase causes male sterility and the gene barstar restores the fertility. Both these genes have been utilized from the bacterium Bacillus amyloliquefaciens.
What is ribonuclease A and function?
Ribonuclease A is a digestive enzyme secreted by the pancreas that specifically “digests” or hydrolyzes RNA (but not DNA) polymers by endonuclease cleavage of the phosphodiester bonds forming the covalent links between adjacent ribonucleotide residues in these molecules.
Is ribonuclease A protein?
This structure includes the dimeric enzyme (in blue) and partial structures for two transfer RNA molecules (in orange). Ribonuclease P, which is composed of both protein components and a ribozyme, trims the other end (not shown).
Which system is a good example of transgenic male sterility?
What are the types of male sterility?
2. Types of Male Sterility:
- Genetic Male Sterility (GMS): The pollen sterility which is caused by nuclear genes is termed as genie or genetic male sterility.
- Cytoplasmic Male Sterility:
- Cytoplasmic Genetic Male Sterility (CGMS):
Why is RNase A used?
RNase A treatment is used for the removal of RNA from genomic DNA samples. RNase A cleaves the phosphodiester bond between the 5′-ribose of a nucleotide and the phosphate group attached to the 3′- ribose of an adjacent pyrimidine nucleotide.